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KMID : 0363320090300010074
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Journal of Korean Oriental Internal Medicine
2009 Volume.30 No. 1 p.74 ~ p.84
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Inhibitory Effect of Injinchunggan-tang(Yinchenqinggan-tang) on Fibrogenesis of Rat Hepatic Stellate Cells
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Lee Hong-Ri
Lee Jang-Hoon
Woo Hong-Jung
Kim Young-Chul
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Abstract
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Objectives: This study was performed to investigate the anti-fibrogenic effect of Injinchunggan-tang on cultured rat hepatic stellate cells.
Materials and Methods: Hepatic stellate cells(HSC-T6) were treated with various concentrations of Injinchunggan-tang extract for 24, 48, and 72 hours. The extraction was done with distilled water. After the treatment, cell viability, proliferation, procollagen levels and the mRNA of the TIMP-1, TIMP-2, and ASMA were measured by using MTT assay. BrdU assay, procollagen type I C-peptide EIA kit and RT-PCR.
Results: The proliferation, mRNA expression and synthesis of collagen of the hepatic stellate cells were inhibited by Injinchunggan-tang treatment in a dose-dependent manner. This indicates the prescription has inhibitory effect on fibrogenesis of the liver by regulating the fibrogenesis associated genes in transcription. Cell viability was inhibited in time- and dose-dependent manners. It seemed that the drug should be used with sufficient dose to acquire treatment effect.
Conclusion: These results suggest that Injinchunggan-tang is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.
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KEYWORD
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Injinchunggan-tang(Yinchenqinggan-tang), liver cirrhosis, liver fibrosis, Hepatic Stellate CeIl(HSC), HSC-T6
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